The new coronavirus variant Omicron has been detected in 13 people who arrived in the Dutch capital Amsterdam on two flights from South Africa.
They are among 61 passengers who tested positive for coronavirus.
It comes as tighter restrictions come into force in the Netherlands, amid record Covid cases and concerns over the new variant.
This includes early closing times for hospitality and cultural venues, and limits on home gatherings.
Omicron was first reported to the World Health Organization (WHO) by South Africa on Wednesday, and early evidence suggests it has a higher re-infection risk. It has been categorized by the WHO as a “variant of concern”.
The Dutch National Institute for Public Health announced the 13 Omicron cases on Sunday, but noted that its investigation had “not yet been completed”, meaning the new variant could still be found in more test samples.
Dutch Health Minister Hugo de Jonge made an “urgent request” for people who have returned from southern Africa to get tested for Covid “as soon as possible”.
“It is not unthinkable that there are more cases in the Netherlands,” he told reporters.
Cases of the heavily mutated variant have now been reported in a number of countries around the world, including several in Europe, such as the UK, Germany and Italy.
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The flights by Dutch national carrier KLM from Johannesburg arrived on Friday morning. The 600 passengers on board were held for several hours after arriving while they were tested for the virus.
New York Times correspondent Stephanie Nolen, who was on one of the flights, tweeted that the passengers were not even brought water while they remained on the plane.
Passengers traveling from South Africa to the UK via Amsterdam told the BBC that they were held on the tarmac at Schiphol airport for four hours, before eventually disembarking.
The passengers who were confirmed to have Covid-19 have since been quarantined at a hotel near Schiphol airport.
Those that tested negative have been asked to isolate at home for five days and take further tests. Officials said those in transit would be allowed to continue their journeys, though there were reports on Saturday that some passengers had not received written proof of a negative test and were therefore unable to board onward flights.
Dutch health authorities were also trying to contact and test thousands of other passengers who had traveled from seven southern African countries – South Africa, Botswana, Eswatini, Lesotho, Mozambique, Namibia or Zimbabwe – since Monday.